描述Thermo Scientific Pierce MMTS (methyl methanethiosulfonate) is a small compound that reversibly blocks cysteines and other sulfhydryl groups, enabling the study of enzyme activation and other protein functions.Features of MMTS (methyl methanethiosulfonate): ? Converts sulfhydryl groups on cysteine side chains into -S-S-CH3 ? Reaction is reversible with DTT or TCEP, restoring the free sulfhydryl ? Used to modify thiol groups in creatine kinase MMTS (methyl methanethiosulfonate) is a sulfhydryl-reactive compound that can reversibly sulfenylate thiol-containing molecules. Reacting MMTS with reduced sulfhydryls (-SH) results in their modification to dithiomethane (-S-S-CH3). Treatment with reducing agents, such as dithiothreitol (DTT), 2-mercaptoethanol (2-ME) or Tris(2-carboxyethyl) phosphine hydrochloride (TCEP), will cleave the disulfide groups to restore the original sulfhydryl. MMTS is commonly used to study biochemical pathways involving thiol-dependent enzymes. |
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