首页>商品中心>试剂/校准液 >化学试剂 >纯化试剂 >商品详情

授权代理商Restore™ PLUS Western Blot Stripping Buffer试剂_500 mL

500 mL

会员价请登录市场价￥2179

原厂型号 46430
贝雷猫货号 RH176076
品牌热电/Thermofisher
单件重量 1kg
货期 7天

单位：瓶包装：瓶库存： 0

数量：瓶 + - 1瓶起订,增量1瓶

加入购物车立即购买

累计成交14笔 0条评价

商品信息商品参数评价(0)

Description

Thermo Scientific Restore PLUS Western Blot Stripping Buffer is an advanced formula for removing bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Features of Restore PLUS Western Blot Stripping Buffer:

• Ready and easy to use—no dilution necessary; no offensive odors; store at room temperature
• Compatible—use on nitrocellulose and PVDF membranes, whether still wet or already dry; works with practically any blocking buffer, enzyme conjugate and chemiluminescent substrate
• Cost effective—save valuable time and samples; strip blots effectively the first time
• Robust yet gentle—transferred proteins remain viable; strip the same blot up to five times
• Flexible—strip and reprobe to optimize antibody concentrations or to detect a new antigen with different antibodies

Restore PLUS Buffer is an alternative formulation of the original Thermo Scientific Restore Western Blot Stripping Buffer. Restore PLUS Stripping Buffer was designed for use with antibodies that are difficult to remove from western blots and require longer incubation times or incubation temperatures greater than 22°C with gentler formulations. High-affinity antibodies can be quickly and effectively stripped from western blots at room temperature without removing transferred proteins, thereby allowing multiple reprobes of the target.

Protocol Summary:
• Wash blot to remove chemiluminescent substrate
• Incubate blot in Restore Western Blot Stripping Buffer for 5 to 15 minutes at room temperature
(or incubate at 37°C for high affinity antibodies)
• Remove blot and wash in Wash Buffer
• Block membrane
• Test for sufficient removal of antibodies
• Perform next immunoblot experiment

无参数