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The ChargeSwitch® gDNA Rendered Meat Purification Kit allows for rapid and efficient purification of genomic DNA (gDNA) from animal feed, animal meal, and heparin products for downstream molecular analysis. The kit is listed in the U.S. Food and Drug Administration Center for Veterinary Medicine’s protocols as a sample preparation method suitable for monitoring crude heparin quality and testing animal feed for banned materials such as specific animal proteins. Note: The number of reactions possible with this kit is dependent upon the starting sample type: animal feed & meal samples = 50 preparations, heparin products = 25 preparations. The ChargeSwitch® gDNA Rendered Meat Purification Kit protocol starts with prepared lysates and takes approximately 15 minutes to complete without centrifugation, vacuum, or organic solvents (for batches of up to 5 samples per operator—larger batch sizes will require longer processing times). The kit employs a magnetic bead with a switchable surface. Low pH conditions cause binding of DNA to the bead while high pH conditions cause the release of the DNA. Tested by the U.S. Food and Drug Administration The ChargeSwitch® gDNA Rendered Meat Purification Kit has been thoroughly tested by the U.S. Food and Drug Administration for processing up to 500 mg of animal feed, 250 mg of animal meal, and 250 mg of heparin product per reaction. References Development of a Multiplex Real-Time PCR Assay for the Detection of Ruminant DNA in Raw Materials used for Monitoring Crude Heparin for Quality www.fda.gov/AnimalVeterinary/ScienceResearch/ToolsResources/ucm350289.htm Development, evaluation, and peer verification of a rapid real-time PCR method for the detection of animal material. Journal of Food Protection, Vol. 72, No. 11, 2009, Pages 2368–2374. U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, Maryland 20708, USA. |
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