描述A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Vitamin A is a customized version of the original B-27 Supplement without vitamin A (retinyl acetate). B-27 Supplement Minus Vitamin A is a serum-free supplement ideal for the cultivation of neural progenitor and stem cells, either as neurospheres in suspension or in adherent monolayer culture, without inducing differentiation. B-27 Supplement Minus Vitamin A is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media. For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook. View publications using Gibco neurobiology products at our Gibco CellCite. |
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