描述Trypan blue staining is a long-standing and widely used method to identify dead cells. Only cells with intact membranes can effectively exclude the dye, so dead cells with compromised membranes become stained. Trypan blue staining is the method employed by the Countess family of automated cell counters, and this 0.4% trypan blue solution is the correct concentration for use on these instruments.Note: Precipitates are quite common in trypan blue, and typically increase with storage time and freezing. The three approaches below may help minimize trypan blue precipitate in your cell counting samples: 1. Sterile filtration of trypan blue using 0.3-micron filter 2. Centrifugation or passive settling (avoid mixing or vortexing trypan stock solutions) 3. Gentle warming of the 1-mL trypan solution to 37°C for ten minutes
台盼蓝是一种长期广泛使用的鉴定死细胞的方法。染料不能对细胞膜完整的细胞进行染色，只能对细胞膜受损的死细胞进行染色。台盼蓝染色法适用于 Countess 系列自动细胞计数仪，在这些仪器上适合使用 0.4% 台盼蓝溶液。 |
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