描述Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.Applications • Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies • Induction of gene expression under control of the lac promoter Note • For blue/white colony screening use 0.1 mM final IPTG concentration in LB (Luria Broth) media • Store IPTG Solution at -20°C. |
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