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Donkey anti-Goat IgG 二抗_1mg

1mg

会员价请登录市场价￥3800

原厂型号 A32814
贝雷猫货号 RH304125
品牌 Invitrogen
单件重量 1kg
货期 7-10天

单位：瓶包装：瓶库存： 0

数量：瓶 + - 1瓶起订,增量1瓶

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产品细节

Applications

Tested Dilution

Publications

免疫细胞化学 (ICC)

1-10 µg/mL

免疫荧光 (IF)

1-10 µg/mL

免疫印迹 (WB)

0.1-0.4 µg/mL

产品规格

种属反应

Goat

宿主/亚型

Donkey / IgG

分类

Polyclonal

类型

Secondary Antibody

抗原

Gamma Immunoglobins Heavy and Light chains

偶联物

Alexa Fluor® Plus 488

激发/发射光谱

查看光谱

形式

Liquid

浓度

2 mg/mL

纯化类型

Affinity chromatography

保存液

proprietary buffer, pH 6.5

内含物

0.016% Methylisothiazolone, 0.016% Bromonitrodioxane

保存条件

4° C, store in dark

RRID

AB_2762838

靶标

IgG

抗体形式

Whole Antibody

产品详细信息

To minimize cross-reactivity, the donkey anti-goat IgG whole antibodies have been cross-adsorbed against IgG from human, mouse, rabbit, and rat, as well as non-immunoglobulin goat serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

Specificity: This antibody binds to heavy chains on goat IgG and light chains on all goat immunoglobulins. This antibody does not bind non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit or rat.

靶标信息

Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Alexa Fluor Plus secondary antibodies provide up to 4.2 fold higher signal-to-background in imaging applications and up to 5.8 fold higher signal-to-background in fluorescent western applications compared to Alexa Fluor secondary conjugates.

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