描述Ambion® ULTRAhyb®-Oligo is specifically formulated for use with oligonucleotide probes in northern analysis. It contains a unique blend of hybridization accelerators and blocking agents that greatly enhance the levels of hybridization so that signals that once took days to visualize are now apparent in hours. It is supplied in one bottle containing 125 mL.• Increase the sensitivity of northern analysis • Use less probe per blot • Decrease hybridization time to just 2 hours Increase Sensitivity Up to 100x or Decrease Hybridization to Just 2 Hours Using standard hybridization buffers, only 1–5% of target molecules on a blot hybridizes to probe, making blots a relatively insensitive method for nucleic acid analysis (Vernier et al. (1996) Anal Biochem 235: 11-19). With ULTRAhyb® Ultrasensitive Hybridization Buffer, the hybridization reaction approaches completion, so that as few as 10,000 molecules can be detected. Oligonucleotides ranging in length from 26 to 45 are used with ULTRAhyb®-Oligo. It works well with oligonucleotides having Tms from 78°–90°C and 47–62% GC contents. However, the optimal hybridization temperature for oligonucleotide probes with different GC content and Tm values should be determined empirically. Accessory Products: The BrightStar® -Plus Positively Charged Nylon Membranes (SKU# AM10100, AM10102, or AM10104) are recommended for use with ULTRAhyb®-Oligo. |
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