描述DNAzol® BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes.• Specifically formulated for extraction of genomic DNA from whole blood • Optimized for the rapid Isolation of genomic DNA Efficient Isolation of Genomic DNA from Whole Blood DNAzol® BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol® BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. 1 ml of DNAzol® Reagent can be used to isolate genomic DNA from 0.5 ml of whole blood to yield 10-20 µg DNA. In addition to the isolation of genomic DNA, DNAzol® BD Reagent can also be used for the isolation of apoptotic fragments from whole blood and viral DNA from serum. Rapid Isolation of Genomic DNA The DNAzol® Reagent combines reliability, efficiency and simplicity in its isolation protocol. In the procedure, blood samples are mixed with DNAzol® Reagent and DNA is precipitated from the resulting lysate with isopropanol. The DNA pellet is then washed successively with DNAzol® Reagent followed by ethanol, and then solubilized. The entire procedure can be completed in about 30 min. Isolated DNA can be Used for a Number of Downstream Applications: Genomic DNA extracted using the DNAzol® procedure is suitable for a variety of applications, including Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization. For Research Use Only. Not intended for animal or human diagnostic or therapeutic use. |
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