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CD11b Monoclonal Antibody (M1/70), eBioscience™（试剂）_100ug

100ug

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原厂型号 14-0112-82
贝雷猫货号 RH304753
品牌 Invitrogen
单件重量 1kg
货期 7-10天

单位：瓶包装：瓶库存： 0

数量：瓶 + - 1瓶起订,增量1瓶

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产品细节

Applications

Tested Dilution

Publications

流式细胞分析 (Flow)

0.25 µg/test

View 334 publications

功能检测 (FN)

Assay-Dependent

View 3 publications

免疫组化（冰冻） (IHC (F))

Assay-Dependent

View 3 publications

免疫沉淀 (IP)

Assay-Dependent

View 1 publication

中和实验 (Neu)

Assay-Dependent

View 5 publications

免疫组化 (IHC)

View 24 publications

免疫荧光 (IF)

View 36 publications

体外检测 (IV)

View 1 publication

免疫细胞化学 (ICC)

View 6 publications

其他PubMed (Misc)

View 6 publications

酶联免疫吸附实验 (ELISA)

View 1 publication

免疫印迹 (WB)

View 1 publication

免疫组化（石蜡） (IHC (P))

View 1 publication

抑制试剂 (IA)

View 1 publication

产品规格

种属反应

Mouse

已发表种属

Fish, Human, Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

M1/70

偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

RRID

AB_467108

产品详细信息

Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.

Applications Reported: The M1/70 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue. The M1/70 clone is not recommended for immunohistochemical staining of formalin-fixed paraffin embedded tissue. M1/70 has also been reported in in vitro blocking of CD11b function. (Please use Functional Grade purified M1/70, cat. 16-0112, in functional assays.).

Applications Tested: The M1/70 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

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