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CD34 Monoclonal Antibody (RAM34), eBioscience™（试剂）_500ug

500ug

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原厂型号 14-0341-85
贝雷猫货号 RH304754
品牌 Invitrogen
单件重量 1kg
货期 28-42天

单位：瓶包装：瓶库存： 0

数量：瓶 + - 1瓶起订,增量1瓶

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产品细节

Applications

Tested Dilution

Publications

流式细胞分析 (Flow)

1 µg/test

View 60 publications

免疫组化（冰冻） (IHC (F))

Assay-Dependent

View 6 publications

免疫组化（石蜡） (IHC (P))

Assay-Dependent

View 3 publications

免疫组化 (IHC)

View 15 publications

免疫荧光 (IF)

View 20 publications

功能检测 (FN)

View 1 publication

免疫细胞化学 (ICC)

View 1 publication

产品规格

种属反应

Mouse

已发表种属

Fish, Goat, Hamster, Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

RAM34

偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

RRID

AB_467211

产品详细信息

Description: The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.

Applications Reported: This RAM34 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections (acetone-fixed only, not formalin-fixed), and immunohistology staining of paraffin embedded tissue sections.

Applications Tested: This RAM34 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (Fluorochrome-conjugated RAM34 is recommended for use in flow cytometry).

Note: When staining with the RAM34 antibody an incubation time of 90 minutes is recommended to obtain the optimal signal to noise ratio.

For more detailed information on staining with RAM34, please refer to Ema et al., listed under References.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

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