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CD45 Monoclonal Antibody (30-F11), eBioscience（试剂）_100ug

100ug

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原厂型号 14-0451-82
贝雷猫货号 RH304756
品牌 Invitrogen
单件重量 1kg
货期 7-10天

单位：瓶包装：瓶库存： 0

数量：瓶 + - 1瓶起订,增量1瓶

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产品细节

Applications

Tested Dilution

Publications

流式细胞分析 (Flow)

0.5 µg/test

View 174 publications

功能检测 (FN)

Assay-Dependent

View 3 publications

免疫组化 (IHC)

Assay-Dependent

View 20 publications

免疫沉淀 (IP)

Assay-Dependent

免疫荧光 (IF)

View 32 publications

免疫组化（石蜡） (IHC (P))

View 5 publications

免疫细胞化学 (ICC)

View 2 publications

免疫组化（冰冻） (IHC (F))

View 8 publications

体外检测 (IV)

View 1 publication

酶联免疫吸附实验 (ELISA)

View 1 publication

产品规格

种属反应

Mouse

已发表种属

Fish, Human, Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

30-F11

偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

RRID

AB_467251

产品详细信息

Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 30-F11 has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified 30-F11 in functional assays.).

Applications Tested: The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

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