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CD8a Monoclonal Antibody (53-6.7), eBioscience™（试剂）_100ug

100ug

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原厂型号 14-0081-82
贝雷猫货号 RH304757
品牌 Invitrogen
单件重量 1kg
货期 7-10天

单位：瓶包装：瓶库存： 0

数量：瓶 + - 1瓶起订,增量1瓶

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产品细节

Applications

Tested Dilution

Publications

流式细胞分析 (Flow)

0.25 µg/test

View 300 publications

免疫组化（冰冻） (IHC (F))

Assay-Dependent

View 7 publications

免疫沉淀 (IP)

Assay-Dependent

View 1 publication

免疫组化 (IHC)

View 22 publications

免疫荧光 (IF)

View 17 publications

功能检测 (FN)

View 13 publications

其他PubMed (Misc)

View 2 publications

免疫细胞化学 (ICC)

View 4 publications

中和实验 (Neu)

View 4 publications

免疫印迹 (WB)

View 1 publication

产品规格

种属反应

Mouse

已发表种属

Bacteria, Fish, Hamster, Human, Mouse, Rat

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

53-6.7

偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

RRID

AB_467087

产品详细信息

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. It has also been reported in T cell activation, isolation and depletion of CD8+ cells, and blocking of ligand binding. (Please use Functional Grade purified 53-6.7, cat. 16-0081, in functional assays.).

Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.

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