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CD3e Monoclonal Antibody (145-2C11), FITC, eBioscience™(试剂)_100ug

100ug

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原厂型号 11-0031-82
贝雷猫货号 RH306167
品牌 Invitrogen
单件重量 1kg
货期 7-14天

单位：瓶包装：瓶库存： 0

数量：瓶 + - 1瓶起订,增量1瓶

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产品细节

Applications

Tested Dilution

Publications

流式细胞分析 (Flow)

0.5 µg/test

View 175 publications

免疫细胞化学 (ICC)

10 µg/mL

View 1 publication

免疫荧光 (IF)

10 µg/mL

View 8 publications

免疫组化（冰冻） (IHC (F))

10 µg/mL

View 2 publications

T细胞活化 (TCA)

View 4 publications

免疫组化 (IHC)

View 4 publications

免疫沉淀 (IP)

View 1 publication

免疫印迹 (WB)

View 1 publication

其他PubMed (Misc)

View 1 publication

功能检测 (FN)

View 2 publications

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

145-2C11

偶联物

FITC

激发/发射光谱

查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.1% gelatin

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

RRID

AB_464882

产品详细信息

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications Reported: This 145-2C11 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 145-2C11 antibody has been tested by immunohistochemistry on frozen mouse spleen and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

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