描述The Qubit RNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay. The assay kit is designed to be accurate for initial RNA sample concentrations from 1 ng/µL to 1 µg/µL providing an assay range from 20–1,000 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer.Which product to choose for RNA BR (Broad Range) quantitation? • For 1–20 samples: use this Qubit RNA BR Assay Kit with the Qubit Fluorometer • For 20–2000 samples: use the Quant-iT RNA BR Assay Kit with microplate reader Notes: 1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers. 2. 500 µL thin-walled PCR tubes are required but not included. |
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