描述Bolt MES SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris gels. It is recommended for separating small- to medium-sized proteins.Use the right buffer to optimize protein separations Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer. Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels See all available buffers and reagents available for SDS-PAGE |
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