描述Knockout™ Serum Replacement (Knockout™ SR) is for serum-free, feeder-based culture of embryonic stem cells (ESCs) and induced Pluripotent stem cells (iPSCs) from multiple species. This defined, serum-free formulation directly replaces FBS in existing protocols.Many of the drawbacks of using FBS in stem cell culture, (differentiation of cells and heat-inactivation) can be alleviated with the use of KnockOut™ Serum Replacement. ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission should not be compromised. With over 2000 peer-reviewed publications covering applications such as stem cell culture, differentiation, iPSC generation, ESC derivation, and transgenic modeling, KnockOut™ Serum Replacement is widely cited for stem cell culture. |
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