描述The Qubit 1X dsDNA BR (Broad Range) Assay kit, designed for use with the Qubit 4 and Qubit Flex fluorometers, provides an accurate and precise method for the quantification of dsDNA samples. The assay is highly selective for dsDNA over ssDNA, RNA, and protein. Additionally, this assay tolerates many contaminants common to nucleic acid isolation and purification workflows, such as salts, solvents, detergents, and free nucleotides.The assay kit is designed to be accurate for initial DNA sample concentrations of 200 pg/μL to 4000 ng/μL (depending on sample volume), providing a detection range of 4–4000 ng of DNA in the assay tube. The '1X' dsDNA BR assay kit provides a ready-to-use working solution. Simply add your sample (any volume between 1 µL and 20 µL), add the Qubit 1X dsDNA BR working solution, and read the concentration using a Qubit 4 or Qubit Flex fluorometer. Notes 1. Use thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 fluorometer and 8 x 200-µL tube strips (Cat. No. Q33252) for the Qubit Flex fluorometer. 2. Qubit 4 and Qubit Flex fluorometers may require a firmware update to run the 1X dsDNA BR Assay. |
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