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孕二醇-3-葡萄糖醛酸 (PDG) 竞争性 ELISA 试剂盒_96 tests

96 tests

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原厂型号 EIAPDG
贝雷猫货号 RH444314
品牌 Invitrogen
单件重量 1kg
货期 28-42天

单位：盒包装：盒库存： 0

数量：盒 + - 1盒起订,增量1盒

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关于本试剂盒

The Pregnanediol-3-Glucuronide (PDG) ELISA quantitates PDG in extracted serum, plasma, urine, dried fecal extracts or cell culture medium.

原理方法

The Pregnanediol-3-Glucuronide (PDG) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure PDG and its metabolites in urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum, plasma, or saliva samples without extraction. The kit will quantitatively measure PDG present in diluted buffer samples and tissue culture media samples. A PDG standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A PDG-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to PDG to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound PDG-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

靶标信息

Pregnanediol-3-Glucuronide (PDG) is a natural human metabolite of Pregnanediol generated in the liver by UDP glucuonyltransferase. Glucuronidation is used to assist in the excretion of toxic substances, drugs or other substances that cannot be used as an energy source. Glucuronic acid is attached via a glycosidic bond to the substance, and the resulting glucuronide, which has much higher water solubility than the original substance, is eventually excreted by the kidneys.

严格验证

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

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