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Mouse IL-18 Uncoated ELISA Kit with Plates（试剂）_10x 96 Tests

10x 96 Tests

会员价请登录市场价￥6115

原厂型号 88-50618-86
贝雷猫货号 RH446057
品牌 Invitrogen
单件重量 1kg
货期 42-56天

单位：盒包装：盒库存： 0

数量：盒 + - 1盒起订,增量1盒

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关于本试剂盒

The Mouse Interleukin-18 (IL-18) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-18. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

原理方法

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

靶标信息

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.

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