The iScript gDNA Clear cDNA Synthesis Kit integrates an effective DNase digestion step with fast and sensitive first-strand cDNA synthesis for accurate qPCR data.
This kit combines the iScript Reverse Transcription Supermix and a specially formulated DNase to effectively eliminate genomic DNA (gDNA) from purified RNA in 10 minutes. qPCR-ready cDNA free of contaminant gDNA is generated in 36 minutes.
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Effective genomic DNA removal — without compromising cDNA synthesis efficiency
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Proprietary DNase buffer — maintaining RNA integrity during gDNA removal
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Convenient all-in-one, single-tube supermix — for reproducible and efficient cDNA synthesis
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Ready-to-use no-RT control supermix provided — for monitoring gDNA contamination
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Superior sensitivity and reproducibility — works with a broad linear dynamic range of input total RNA (1 µg–1 pg)
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Unbiased qPCR data — consistent efficiency, sensitivity, and reproducibility in qPCR results even after gDNA removal
gDNA-free cDNA ready in 36 minutes
This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.
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