首页>商品中心>试剂/校准液 >化学试剂 >纯化试剂 >商品详情

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5)试剂_100ug

100ug

会员价请登录市场价￥1283

原厂型号 14-5982-82
贝雷猫货号 RH459952
品牌 Invitrogen
单件重量 1kg
货期 3-7天

单位：瓶包装：瓶库存： 0

数量：瓶 + - 1瓶起订,增量1瓶

加入购物车立即购买

累计成交1笔 0条评价

商品信息商品参数评价(0)

产品详细信息

Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Applications Reported: The MIH5 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking in in vitro and in vivo functional assays. (Please use Functional Grade Purified MIH5, cat. 16-5982, in functional assays. Fluorochrome-conjugated MIH5 is recommended for use in flow cytometry.).

Applications Tested: This MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

无参数