Omni-Rapid™快速蛋白定量试剂盒
Omni-Rapid™Protein Assay Kit
本产品冰袋运输; 即用型BSA标准品 -20℃保存,其它组分4℃保存,保质期12个月。
货号规格
goodZJ103 500次(微孔)
产品特点
g 简单快速——室温5 min完成显色反应;
g 方便快捷——提供即用型标准品,省去繁琐的稀释步骤;
g 准确性高——变异系数远小于考马斯亮蓝染色法;
g 线性范围宽——灵敏,检测范围:20~2000 μg/mL;
g 兼容性好——与大多数金属离子、螯合剂及去污剂兼容性较好。
产品简介
goodOmni-Rapid™快速蛋白定量试剂盒的原理与传统BCA蛋白定量法类似,但采用了一种不同于BCA(Bicin-choninic Acid)的全新特殊的螯合剂,从而实现了对蛋白质浓度进行快速、稳定、灵敏的测定。其原理是在碱性环境下蛋白质分子中的肽键能与Cu2+形成络合物,将Cu2+还原成Cu+,Cu+与螯合物结合,从而发生颜色反应。本试剂盒中的螯合剂可敏感特异地与Cu+结合,只需室温孵育5 min即可形成稳定的橙黄色水溶性复合物,而传统BCA法则需在37℃下孵育30 min才可完成颜色反应。该橙黄色的复合物在480 nm处有强光吸收值,颜色的深浅与蛋白质浓度成正比,可根据吸收值的大小来测定蛋白质的含量。本试剂盒含有一系列浓度的蛋白质标准品溶液(BSA溶液),即取即用,无需稀释,方便快捷。
相关论文
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