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C57BL/6 mouse splenocytes were stimulated for 72 hours with CD3e and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0031-85) and (Product # 16-0281-85), followed by Protein Transport Inhibitor Cocktail (Product # 00-4980-03), for an additional 5 hours. Cells were then surface stained with CD8a Monoclonal Antibody, FITC (Product # 11-0081-82) followed by intracellular staining of 0.06 µg of Rat IgG2a kappa Isotype Control, PE-Cyanine7 (Product # 25-4321-82) (left) or 0.06 µg of Granzyme B Monoclonal Antibody, PE-Cyanine7 (right)... View More
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