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Thermo Scientific Pierce 8M Guanidine-HCl Solution is an accurately formulated, ready-to-use solution of guanidine hydrochloride, that can be easily diluted and pH-adjusted to any concentration below 8M. Features of 8M Guanidine-HCl Solution: • Any concentration below 8M guanidine can be quickly and easily prepared. • No wasting valuable research time weighing and dissolving guanidine crystals • Free from UV absorbing materials in the range of 225-300nm • Free from heavy metal contaminants • Free from degradation products such as ammonia • Excellent stability Applications of Guanidine Hydrochloride: • Solubilizing proteins from inclusion bodies • Increasing solubility of hydrophobic peptides and proteins • Denaturing proteins Chaotropes, substances that disrupt the structure of water interactions, help to solubilize hydrophobic proteins and peptides for a variety of biological purposes. For example, hydrophobic peptides and proteins may be dissolved in coupling buffer containing either 6M guanidine-HCl or 4M urea prior to immobilization on AminoLink Plus Support to create an affinity column. However, because the degradation products of guanidine and urea include ammonia, which contains a primary amine that will compete for reaction to the support, urea and guanidine solutions must be prepared with high quality reagents immediately before use. Guanidine is a general protein denaturant, unfolding proteins and altering their three-dimensional structure. Consequently, some proteins will be irreversibly altered upon interaction with guanidine solutions and may lose their binding function. Before any large-scale use of guanidine, it is best to test a small sample and determine whether the denaturing effects will adversely affect the intended use of the protein. Related Products Guanidine-HCl |
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